Characteristics
The gene delivery vector(carrier) of Xylifectamine(PdXYP) and can be used as a gene delivery system for research only. The cell viability of this vector is ranged from 95% to 100% in normal cells, so the vector has low cytotoxicity (Fig. 1). note: VBXYP: Vit. Bx-coupled PdXYP
The gene delivery vector(carrier) of Xylifectamine(PdXYP) can be used as a gene delivery system for research only. The cell viability of this vector is ranged from 98% to 100% in cancer cell lines, so the vector has low cytotoxicity (Fig. 2).
The transfection efficiency of Xylifectamine vector is ranged from 55% to 70%, depending on cell lines. The transfection efficiency is higher than those of other polymer vectors (Fig. 3).
Xylifectamine vector based on a xylitol polymer & PEI cause caveolae-mediated endocytosis on the cell membrane. So its cellular uptake is high.
Xylifectamine is much absorbed into spleen, lung, brain, kidney, liver and heart in order in the biodistribution imaging test of mice (Fig. 4).
Xylifectamine vector is so hyperosmotic that it well transmigrates blood brain barrier(BBB) and blood tumor barrier(BTB) by NFAT5 protein transporter caused for osmotic balance on the cell membrane and by loosened tight junctions between cells.
Xylifectamine vector with (+) charge wraps and condenses genes with (–) charge. So it makes nano particles. And the genes inside of nano particles are protected from RNA & DNA lyases and are stable in serum or blood.
The N/P molar ratio of vector VS genes is recommended by 20:1, and its weight ratio is 8:1, where the concentration of Xylifectamine vector is 10 mg/ml, the concentration of DNA is 1 mg/ml. The vector with lower N/P ratio(15~20:1) can be used for gene delivery even though its transfection efficiency decreases a little.
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